Developing ‘genetic barcodes' for monitoring individual colonies and tracking their offspring
The CRISPR-Cas9 genome editing system can be used to create genetic barcodes in individuals. This can be done by creating random mutations in a corals DNA that have no effect on the animal – similar to a tag. These sites of random mutations represent a unique genetic barcode that can be used not only to track the individual over its lifetime but potentially also its offspring. If this research shows the approach is viable, it is envisaged it could be co-developed with an eDNA detection capability to provide an essential tool for restoration and adaptation management.
Monitoring of these genetic barcodes can be merged with the above general genetic diversity monitoring to track coral at both the individual colony and population level. This would be extremely valuable for informing coral reef management and restoration, as it can give insight into how out-planted (barcoded) coral colonies are surviving, spreading, and integrating into the natural population and how general genetic diversity is changing in response to restoration efforts.
Developing a rapid test for coral heat tolerance using CRISPR technology
We aim to develop a way to test a corals' heat tolerance by measuring their gene expression in response to acute heat stress.
By observing how a coral upregulates (or increases activation of) certain genes in response to heat stress, we can predict its heat tolerance. When a coral upregulates a gene, it increases the amount of RNA produced by that gene. We can use CRISPR systems to measure RNA for specific genes, which can be visualised using a marker, similar to commonly used rapid RNA tests for COVID-19. This approach would provide a holistic, low-cost, high-throughput method for screening heat tolerance in corals.
CRISPR/Cas9 research to date
Over the past five years, we have developed technology to make precise changes in the coral genome using CRISPR/Cas9.
- In 2018, we identified a key gene, HSF1, that controls coral tolerance to heat stress. This is the first gene to be shown to control coral survival to heat.
- In 2020, we identified a gene we believe is involved in corals' ability to biomineralise their skeletons.
- From 2021, we have built a database of key genes for CRISPR screens from several Acropora coral species, to increase the knowledge of the genetic basis of heat stress in corals and feed the possibilities of CRISPR screens.
- In 2022 and 2023, we continued to identify genes that control survival to acute heat stress and have explored how the regulation of these genes effect survival over time and across species.
Researchers
Dr Line Bay, AIMS
Dr Phillip Cleves, Carnegie Institution for Science
Dr Luke Thomas, AIMS
Max Moonier, AIMS and UWA
Dr Severine Fourdrilis, AIMS
Dr Ryan Lister, UWA
This research is supported by
Carnegie Institution for Science
Australian Institute of Marine Science
The European Union’s Marie Skłodowska-Curie Actions
University of Western Australia
This page was updated in October 2024