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Marine 
biotechnology group

The Marine Biotechnology Group leads in the adoption of integrative strategies for the sustainable use and protection of marine resources, detailed most recently in the Review of Marine Research in Tropical Australia (July 2001). Priorities in 2001-02 were improving production and culturing new targets for global markets, tapping genetic resources for new pharmaceutical and healthcare products, trialing marine compounds effective as agrichemicals for crop protection, and developing bioremediation agents for environmental protection.

The Group strives to achieve a balance between applied research geared toward commercial applications, and basic research centred on preserving marine biodiversity and the environment. In this capacity, it contributed to international forums and the national agenda to develop Commonwealth and State policies on access to biodiversity and benefit-sharing. In September 2001, the Group also provided habitat information and collection inventories from southern Australian sites to the National Oceans Office Uses Assessment for the development of the Southeast Regional Marine Plan. The Institute’s ability to generate industry from biodiversity was recognised in the Queensland Biodiscovery Policy Discussion Paper (April 2002). 

Developing aquaculture technologies

Drs. Cherie Motti and Dianne Tapiolas check for antibiotic activity

Tropical aquaculture output focused on prawn domestication and genetics, the culturing of new targets including lobsters and sponges, and advising industry on production. The Tropical Aquaculture Project succeeded in a consortium bid with two divisions of CSIRO and the Queensland Department of Primary Industries for a $5.5 million project funded by the Fisheries Research and Development Corporation (FRDC) and Industry, looking at Understanding and removing the barriers to Penaeus monodon domestication. AIMS benefits by $378,000 for infrastructure and operating costs over three years. 

-Drs. Cherie Motti and Dianne Tapiolas
       check for antibiotic activity

This collaborative research program is the largest of its kind in history and brings together industry partners and the four major agencies conducting research in tropical aquaculture. It is receiving major support from the Australian Prawn Farmers Association (APFA) and leading farm enterprises.

The Institute’s contribution in developing a prawn broodstock management policy has also gained industry backing from the APFA and Queensland Fishing Industry Development Council, and the provisional patent Induction of spawning in Crustacea has progressed to a national-phase application.

The sea-ranching of sponges for fine chemicals and fibres (e.g. collagen for bone and wound healing, cosmetics, bath sponges) attracted community interest in 2001-02. Scale-up research was undertaken with support from FRDC and the Queensland and Western Australian Governments. Outcomes indicate production technologies are sustainable and economic. Queensland-based research was successful, moving to full external funding with $80,000 confirmed from the Department of Aboriginal and Torres Strait Islander Policy and the Great Barrier Reef Research Foundation. Research on sponge aquaculture in Western Australia funded by FRDC ($352,000 over two years) was completed with two species identified as aquaculture targets. The compounds from these species have been placed on the U.S. Food and Drug Administration register for clinical trials with the National Cancer Institute. AIMS is positioned to capitalise on this development, but lack of a biodiversity access agreement with Western Australia has hindered efforts to develop a viable aquaculture industry for the supply of these drug leads in that State.

Scientists look for useful compounds

Corals' gene flow investigated

The search for bioactive molecules from the marine environment continues to bear fruit. 

The Institute maintains a comprehensive and diverse collection of Australasian marine biota curated with a growing informatics database; this provides the framework for the discovery of active compounds with commercial application and scientific value. In 2001-02, more than 8,000 marine macro-organisms were tested for antibiotic activity against E. coli, and the search is being extended to screen for anti-infectives against Staphylococcus aureus and Candida albicans. 

-Corals' gene flow investigated

Additionally, a project with James Cook University funded by Nufarm Limited, the world’s ninth largest agrichemical company, advanced discoveries of a novel herbicide to the glasshouse testing stage, thus achieving a milestone in the transfer of Intellectual Property to industry. Nufarm reported in 2001: "Preliminary results are very encouraging. The most promising candidate molecules so far identified are in synthesis development at Nufarm’s French laboratories and indicate that these organisms could be a rich source of novel crop protection products."

AIMS continues to host the Australian Research Network for Algal Toxins (ARNAT) which brings together more than 100 researchers in Australia, New Zealand, Canada and the US.

Genetics research aids resource management

Genetics is used at AIMS to understand the evolution and maintenance of marine biodiversity. In the project Genetics for Bioresource Management and Ecology, a range of DNA traits were studied in 2001-02 to understand reproductive connections between populations, genetic diversity within marine populations, and the processes leading to this diversity. The use of biotechnology in this manner aids the design and management of Marine Protected Areas. For example, genetic data reveals that cross-fertilisation between coral species is important on an evolutionary timescale, but occurs infrequently in common corals of the genus Acropora. Hence, the effects of this cross-fertilisation are negligible and most species are distinct genetic entities. Population studies on commercially fished holothurians (sea cucumbers or bęche-de-mer) highlighted the need to consider gene flow (genetic exchange between populations) in planning biodiversity protection. Research found that one species with unrestricted gene flow between populations can be managed over large scales, whereas another species with a restricted gene flow requires protection of local populations. A forensic method was developed for identification of specimens to determine growth and migration rates, and this technique is amenable to other species for future conservation planning.

Scientists at AIMS are also using molecular indicators to quantify variations in heat stress responses of coral species during bleaching episodes on the Great Barrier Reef. Like other organisms, corals produce heat shock proteins for protection, and these proteins indicate to scientists the degree of heat stress experienced. In 2001-02, scientists discovered that Acropora millepora coral from an inshore reef was more able to produce these protective proteins and can withstand heat stress more than specimens living on an offshore reef. This thermal tolerance is also correlated with the strain of symbiotic algae or zooxanthellae living in the coral tissue. Results suggest the ability of corals to associate with different strains of symbiotic algae may contribute to their susceptibility to coral bleaching.

Nature’s defences offer new possibilities

Dr Andrew Negri in Antarctica

The search for new biochemicals is complemented by examining adaptations of marine organisms to harmful or aggressive environments resulting in the production of biotoxins and venoms, antifoulants, signalling agents, and other molecular defences. Understanding the functioning of these agents provides a strategy for the discovery of new and useful products.

In 2001-02, the discovery of a novel response by marine bacteria to ultraviolet radiation prompted new research aimed at shedding insight into cellular processes of human ageing and the design of therapeutic controls to retard degenerative processes. 

-Dr Andrew Negri in Antarctica

Marine bacteria isolated from the mucus of shallow-water corals have a biochemical adaptation that enhances the recycling of coenzyme Q (CoQ) in their metabolism. In human health, CoQ is vital to energy production and antioxidant balance. AIMS research into CoQ production by marine bacteria won support from the Australian Academy of Science and the Japan Society for the Promotion of Science. The Institute is now collaborating with the University of Tokyo and the Osaka City University Medical School to take this research to the next level.

In a similar vein, collaboration with the University of Canterbury and logistic support from Antarctica New Zealand underpinned AIMS research into human impacts on Antarctic sponges. The seabed environment of the Antarctic shelf under ice is dominated by sponges which filter hundreds of litres of seawater per hour, making them ideal bio-indicators of pollution near areas of human occupation. Additionally, these animals harbour bacteria with potential to act as indicators of metabolic stress. AIMS researchers examined the effects of pollution on the sponges’ biochemistry and the bacteria they host. This collaboration succeeded in gaining second-year funding from Antarctica New Zealand and is now attracting the interest of environmental management agencies. It provides a novel opportunity to examine tropical-polar extremes in comparative physiology, environmental biochemistry and the functional ecology of marine sponges.

 

RESEARCH Output at a glance – MARINE BIOTECHNOLOGY

  • Start-up company ToxiTech Pty Ltd established with major focus on biotechnological solutions for quality management of seafood toxins and drinking water.

  • Discoveries of novel marine herbicides advanced to glasshouse testing stage.

  • Major consortium bid succeeded in launching $5.5 million project looking at Understanding and removing the barriers to Penaeus monodon domestication.

  • Discovery of a novel response by marine bacteria to ultraviolet radiation prompted new research aimed at shedding light on human ageing and design of therapeutic controls to retard degenerative processes. International collaboration will help take this discovery to next level.

  • AIMS contributed to bioinformatics database for new CD-ROM on Cleveland Bay, Townsville.

  • 8,000 marine macro-organisms tested for antibiotic activity against E.coli. This was the largest discovery effort in Australasia for such biological activity. Leads are now under investigation.

  • DNA traits of marine organisms examined to inform design of Marine Protected Areas. Scientists shed insight on reproductive connections between populations, genetic diversity within marine communities, and the processes leading to their evolution.

  • Population studies on commercially fished holothurians (sea cucumbers) highlighted need to consider gene flow in planning biodiversity protection.

  • Molecular indicators showed heat stress responses differ among coral specimens. Scientists discovered adaptations of algae living in coral tissue to environmental change may be critical to coral bleaching.

  • Scale-up research shows viability of developing sponge aquaculture for the extraction of fine chemicals and fibres.

  • Research into human impacts on Antarctic sponges gained second-year external funding from Antarctica New Zealand and attracted interest of environment management agencies.

  • Habitat information and collection inventories from Southern Australian sites were given to National Oceans Office.

  • Group continued to provide major input to Commonwealth and State Policies on access to biodiversity and benefit-sharing.

 

 

 

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