A centipede found in many Townsville backyards might hold
the key to protecting shellfish fanciers from a chemical so
deadly that the United Nations has banned it.
Saxitoxin, the poison that causes paralytic shellfish
poisoning or PSP, is so potent the UN Chemical Weapons
Convention lists it among the leading chemical weapons of war.
This forced the banning of much research until recently.
AIMS scientist Dr Lyndon Llewellyn became interested in
saxitoxin, which kills hundreds of people worldwide every
year, while studying in the United States, although he had to
suspend his work until the research ban was lifted.
Dr Llewellyns work involves finding a protein able to
detect saxitoxin so he could develop a cheap, reliable test
kit. Now a garden centipede is his most treasured pet.
"Because PSP is so deadly I wanted to find a way to
detect saxitoxin before people consumed the poisoned
shellfish," he said.
"A garden centipede, found in most backyard compost
heaps, contains the protein I needed to continue my
research," he said.
Saxitoxin, found in a variety of molluscs and crabs that
have been exposed to water-borne algal blooms, attacks the
nervous system but does not affect the brain. The victim is
fully aware of what is happening, but as the name suggests,
becomes paralysed.
"Up to 40 per cent of people contracting PSP die, some
within two hours, because the toxin eventually paralyses the
diaphragm" said Dr Llewellyn.
World health organisations suggest that less than one part
per million of toxin per 100g of shellfish meat will kill an
average human. In the Philippines, children have died from as
little as 0.4 ppm.
Dr Llewellyn suggests cultural differences are a
contributing factor in people contracting PSP.
"People in the worst affected regions, Alaska,
Chile, and the Philippines, gather a lot of their seafood,
placing themselves at risk.
"The toxin is heat stable and water soluble, and
people from these countries are known to have died from
concoctions of seafood made into a soup," he said.
Australia has no deaths reported from PSP; however there
have been two minor cases recorded in Tasmania. Dr Llewellyn
is sceptical about there being only two cases in Australia. He
believes a lot of cases are unreported and treated as an
allergy.
A family of crabs native to Australia and common on the
Great Barrier Reef can be as deadly to humans as any large sea
animal. The green shawl crab is only 3cm across the carapace,
but can have enough saxitoxin to kill 3,000 people.
Shellfish, both farmed and free range, accumulate saxitoxin
after consuming algal bloom while feeding.
Dr Llewellyn said algal blooming is not restricted to
seawater and can happen in fresh water, thus rendering
freshwater shellfish toxic.
Because PSP tends to affect people in poorer countries Dr
Llewellyn saw the need for a portable test kit that was cheap
and easily accessible worldwide.
"When researching for the protein I tested amphibians,
fish and reptiles without much success. Plenty had the protein
but it did not have the properties to be commercially
viable," he said.
Out of frustration, Dr Llewellyn decided to go terrestrial,
beginning with the garden centipede. "I was turning my
compost heap; a centipede stuck its head up so I took it and
tested it."
His research has shown that five centipedes contain enough
protein to test 30,000 samples for PSP.
Dr Llewellyns test will be as simple as taking a sample
from shellfish and placing it on a card, where a colour code
will indicate toxicity immediately.
The kit will also be of value to commercial operations
around the world. Countries that export shellfish are forced
to monitor for toxicity because of the consequences for their
economy should they export toxic products. New Zealand alone
spends $1 million each year on monitoring.
Australia imported $13 million worth of molluscs during the
past financial year, mostly from New Zealand.
"The only authorised testing method at the moment is
by injecting mice, which is barbaric," Dr Llewellyn said.
"Those tests, conducted in a laboratory, cost $200 to
$300 each. My test kit will cost about $1 for material and is
portable."
For further information, please
contact:
Dr Lyndon Llewellyn, AIMS Bioproducts research
project
PH: 07-4772 7707 or mobile 0408 441125
E-mail: